Coding

Part:BBa_K3031002:Design

Designed by: David Doyle   Group: iGEM19_SUIS_Shanghai   (2019-10-13)
Revision as of 05:16, 13 October 2019 by Registry (Talk | contribs)

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CwA (194aa) - Truncated cell wall anchoring protein from Lactobacillus plantarum truncated sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Removal of illegal restriction sites.

The 194 amino acids that make up this truncated protein was obtained from the whole protein sequence and was counted backwards from the N-terminal side of the whole cell wall anchor.


Source

The full protein sequence was obtained from the Lp_2578 gene annotation page on NCBI: https://www.ncbi.nlm.nih.gov/gene/?term=lp_2578.

Information for truncated protein size was obtained from the paper "Recombinant Lactobacillus plantarum induces immune responses to cancer testis antigen NY-ESO-1 and maturation of dendritic cells" Moberglisen et al., (2015).

References