RNA
Part:BBa_K2923001
Designed by: claire husser Group: iGEM19_Strasbourg (2019-09-12)
Theophylline actif aptazyme
an aptazyme is an aptamer-ribozyme combinations with intrinsic RNA cleavage activity. this aptazyme is specific for the theophylline (liguant). Theophylline (1,3-dimethylxanthine) is a drug used in therapy for respiratory diseases and is structurally similar to theobromine and caffeine He is composed in 3 differents part, a twister ribozyme module, a communication module and an aptamer module This aptazyme is a OFF-switches aptazyme, this mean that in the presence of liguant the RNA does not self-cleave and in the absence of liguant the RNA self-cleaves. This mean that you can sense the theophylline in a medium with this aptazyme.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//classic/rna/uncategorized
ligands | Theophylline |
switch_point | 2.5 mM of Theophylline |