Coding

Part:BBa_K3037001:Design

Designed by: Arnau Pérez Roig   Group: iGEM19_TU_Dresden   (2019-10-03)
Revision as of 15:43, 3 October 2019 by Registry (Talk | contribs)

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Maltose Binding Protein (MBP-tag)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 381
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 79


Design Notes

Tagging (removable tag) Affinity chromatography Purification Improvement of yield of recombinant proteins (increase expression and increase solubility) Improvement of solubility of recombinant proteins (solving problem of inclusion bodies) Can help expression of difficult proteins like Cas9 and fusion proteins



Source

Synthesized by Integrated DNA Technologies (IDT)

References