Composite

Part:BBa_K3120020

Designed by: Shuiqin Gong   Group: iGEM19_BM-AMU   (2019-08-14)
Revision as of 07:53, 14 August 2019 by Registry (Talk | contribs)

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pCAG+Twist1-ERT2+IRES+Zeocin ORF

Twist1-Ert2 fusion protein was highly expressed by CAG promoter, and when tamoxifen was added, twist1-ert2 fusion protein nucleated and promoted EMT-related genes.The quantitative control of EMT process was realized by controlling tamoxifen concentration.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 629
    Illegal NgoMIV site found at 2065
    Illegal NgoMIV site found at 2126
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 660


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Parameters
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