Coding
P20 with

Part:BBa_K2938008

Designed by: Assaf Vital   Group: iGEM19_BGU_Israel   (2019-09-28)
Revision as of 12:34, 28 September 2019 by Assafi (Talk | contribs)


P20 with His tag

P20 chaperone with Strep-tag attached in the C- terminal in order to be able to detect the translated protein in western blot (using antibodies against the tag)

Usage and Biology

The P20 is a chaperone which increases the production of Cry11Aa toxin, increases the larvicidal effect on mosquitos’ larvae, and His-tag is used to detect proteins in western blot using antibodies specific to the tag.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 264
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 264
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 264
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 264
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 264
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None