Coding

Part:BBa_K2963020:Design

Designed by: Experiment group members of JNU-China   Group: iGEM19_JNU-China   (2019-09-12)
Revision as of 08:02, 12 September 2019 by Registry (Talk | contribs)

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pgsA - encoding a submit of poly-γ-glutamic acid synthetase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 364
    Illegal PstI site found at 650
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 364
    Illegal PstI site found at 650
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 364
    Illegal PstI site found at 650
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 364
    Illegal PstI site found at 650
    Illegal AgeI site found at 1008
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When we express the CapA protein heterologously in corynebacterium glutamicum, we add Ptac promoter and a short RBS, which can be recognized by corynebacterium glutamicum. And simultaneously, we add T7 terminater used for stopping transcription in time.



Source

Bacillus Genome.

References