Composite

Part:BBa_K3245012

Designed by: Dehao Zhu   Group: iGEM19_Fudan   (2019-10-06)
Revision as of 11:58, 6 October 2019 by Registry (Talk | contribs)

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Part constructed to characterize part R0040

As we use tetR-ptetR system in our project, it is essential for us to measure the minimum amount of tetR needed to stop ptetR. Considering that ptetR is a rather strict regulatory promoter, we constructed 4 tetR-ptetR-GFP expression systems,each with different strength of tetR. Then we measured both their initial GFP strength as well as their induction curve

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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