Part:BBa_K3034000

_NOTOC__ CrpP：Ciprofloxacin-Modifying Enzyme

CrpP is a novel ciprofloxacin-modifying enzyme which can phosphorylate ciprofloxacin(CIP).Then the phosphorylated CIP goes through multiple steps of degradation spontaneously and produces 1,4-dihydroquinoline finally[1]. 1,4-dihydroquinoline is used as a kind of new carriers for specific brain delivery [2], so we can infer that it's nontoxic. This part is responsible for degradation of ciprofloxacin in our project.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Characterization

Molecular weight

This gene codes for a protein of 65 amino acids with a molecular mass about 15 kDa.

References

[1]Chávez-Jacobo, Víctor M., et al. "CrpP is a novel ciprofloxacin-modifying enzyme encoded by the Pseudomonas aeruginosa pUM505 plasmid." Antimicrobial agents and chemotherapy 62.6 (2018): e02629-17. [2]Foucout, Léna?g, et al. "Synthesis, radiosynthesis and biological evaluation of 1, 4-dihydroquinoline derivatives as new carriers for specific brain delivery." Organic & biomolecular chemistry 7.18 (2009): 3666-3673.