Coding
CMYC 6HIS

Part:BBa_K3038000

Designed by: Marine LAVAUD   Group: iGEM19_Poitiers   (2019-10-08)
Revision as of 08:56, 11 October 2019 by Bvannier (Talk | contribs) (→‎Protein Sequence)


Alcohol dehydrogenase - Geobacillus stearothermophilus cMYC and 6His N-terminal tags

ADR : GenBank: P42327

https://www.ncbi.nlm.nih.gov/protein/P42327

Alcohol dehydrogenase; Short=ADH or ADR

===Thermophilic NAD+-dependent alcohol dehydrogenase===. Bears mainly an ethanol-dehydrogenase activity.

Reaction

a secondary alcohol + NAD+ = a ketone + H+ + NADH EC:1.1.1.1

Protein Sequence

M===HHHHHH===KAAVVNEFKKALEIKEVERPKLEEGEVLVKIEACGVCHTDLHAAHGDWPIKPKLPLIPGHEGVGIVVEVAKGVKS IKVGDRVGIPWLYSACGECEYCLTGQETLCPHQLNGGYSVDGGYAEYCKAPADYVAKIPDNLDPVEVAPILCAGVTTYKALK VSGARPGEWVAIYGIGGLGHIALQYAKAMGLNVVAVDISDEKSKLAKDLGADIAINGLKEDPVKAIHDQVGGVHAAISVAVN KKAFEQAYQSVKRGGTLVVVGLPNADLPIPIFDTVLNGVSVKGSIVGTRKDMQEALDFAARGKVRPIVETAELEEINEVFER MEKGKINGRIVLKLKED



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None