Coding
Part:BBa_K3195003
Designed by: JingYuan Liu Group: iGEM19_SEU-Nanjing-China (2019-09-23)
His-tagged Cathepsin B
This Cathepsin B from Branchiostoma belcheri tsingtauense is originally a kind of hydrolase in lysosome.In our project,it has been used to biodegrade microcystin,a kind of biotoxin synthesized by cyanobacteria.
Usage and Biology
File:T--SEU-Nanjing-China--Bronze Characterization.docx
Characterization
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 369
Illegal XhoI site found at 958 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 463
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//cds/enzyme
//chassis/prokaryote/ecoli
//function/degradation
//chassis/prokaryote/ecoli
//function/degradation
origin | Branchiostoma belcheri tsingtauense |
tag | 6X His |