Coding
OriphiC31

Part:BBa_K3254018

Designed by: Yeqing Zong   Group: iGEM19_GENAS_China   (2019-10-10)
Revision as of 09:03, 10 October 2019 by Registry (Talk | contribs)

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original sequence of phiC31 integrase gene

This sequence was placed before the integrase coding sequence in the phiC31 phage genome. Because there is an start codon in the upstream, some researchers likely annotated it as a part of the coding sequence. Here we used this part for achieving a good response interval of phiC31 integrase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 75
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1689
    Illegal SapI.rc site found at 1380
    Illegal SapI.rc site found at 1438
    Illegal SapI.rc site found at 1543


[edit]
Categories
//cds
//chassis/prokaryote/ecoli
//function/recombination
Parameters
None