Coding
T18 domain

Part:BBa_K1638004

Designed by: Jens Sivkr Pettersen   Group: iGEM15_SDU-Denmark   (2015-05-10)
Revision as of 18:28, 8 July 2015 by Jpettersen (Talk | contribs)

T18 domain of CyaA from Bordetella pertussis

This part codes for the T18 domain of the catalytic domain of the adenylate cyclase, CyaA, from Bordetella pertussis. When associated with the T25 domain of the catalytic domain of CyaA, the two domains together become active and catalyzes the conversion of ATP to cAMP. The rise in cAMP can in turn be used to trigger the expression of certain genes by using a cAMP-induced promoter.

The T25 domain can be found here: BBa_K1638002

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 550
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 91
    Illegal NgoMIV site found at 501
    Illegal AgeI site found at 307
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None