Composite
Part:BBa_K3128025
Designed by: Melodie Dupre Group: iGEM19_Grenoble-Alpes (2019-09-21)
COMP fused with T25 subpart of Bordetella Pertussis AC under lactose promoter
This biobrick is composed of the external membrane OMPx protein with Y121F' mutation fused at its N-terminal end with a 54 aa GGS linker (BBa_K3128010) followed by the T25 subunit of the Bordetella Pertussis adenylate cyclase. This biobrick is under plac promoter in order to be activated when the cAMP messenger is present in the system.
This biobrick has a TAG codon (Y121F' mutation) which can be used to introduce any Non Natural Amino acid outside the cell (more informations:LIEN!!).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1568
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 179
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Categories
Parameters
None |