Composite

Part:BBa_K3128025

Designed by: Melodie Dupre   Group: iGEM19_Grenoble-Alpes   (2019-09-21)
Revision as of 14:42, 21 September 2019 by Registry (Talk | contribs)

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COMP fused with T25 subpart of Bordetella Pertussis AC under lactose promoter

This biobrick is composed of the external membrane OMPx protein with Y121F' mutation fused at its N-terminal end with a 54 aa GGS linker (BBa_K3128010) followed by the T25 subunit of the Bordetella Pertussis adenylate cyclase. This biobrick is under plac promoter in order to be activated when the cAMP messenger is present in the system.

This biobrick has a TAG codon (Y121F' mutation) which can be used to introduce any Non Natural Amino acid outside the cell (more informations:LIEN!!).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1568
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 179


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Categories
Parameters
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