Composite

Part:BBa_K3111102

Designed by: Matas Deveikis   Group: iGEM19_UCL   (2019-09-10)
Revision as of 15:33, 10 September 2019 by Registry (Talk | contribs)

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T. maritima encapsulin with a StrepII-tag expressed under T7 promoter

Modification to Team EPFL 2018 part BBa_K2686001 with addition of a StrepII tag to purify assembled encapsulin without heat treatment.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 183
    Illegal BglII site found at 547
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 962
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 481
    Illegal SapI.rc site found at 512


[edit]
Categories
Parameters
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