Coding

Part:BBa_K3036006

Designed by: Di Huang   Group: iGEM19_BNU-China   (2019-10-08)
Revision as of 10:26, 8 October 2019 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


GFP with degradation conduction tag RepA

We design a device for increasing the degradation rate of green fluorescent protein (GFP) by adding a 16-amino-acid-long tag replication protein A (RepA) at the N-terminal, therefore the green fluorescence will degrade sooner when expression ends. Hence, when GFP is used as a reporter, it is more sensitive in monitoring the state of any inducible promoter. In our project, the RepA-GFP part is used in the report plasmid to present the state of the promoter flanked by recombination sites in a more sensitive way, in order to test our bilateral switch. It enlightens us to make the same improvement with our recombination directionality factor (RDF), so as to raise efficiency of the bilateral switch.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 692


[edit]
Categories
Parameters
None