Composite
PLac_LZ-Om

Part:BBa_K3128022

Designed by: PINERO Lucas   Group: iGEM19_Grenoble-Alpes   (2019-09-18)
Revision as of 18:29, 18 September 2019 by Registry (Talk | contribs)

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OmpX-WT with Leucine Zipper and T18 subpart of Bordetella Pertussis AC under lactose promoter

This biobrick is composed of the external membrane OMPx WT protein fused at its N-terminal end with a 54 aa GGS linker (BBa_K3128010) followed by the T18 subunit of the Bordetella Pertussis adenylate cyclase. This biobrick is under plac promoter in order to be activated when the cAMP messenger is present in the system. Leucine zipper are known to interact by forming homodimers. This biobrick is a part of a positive control for the BACTH assay.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1632
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1173
    Illegal NgoMIV site found at 1583
    Illegal AgeI site found at 1389
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 344


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Categories
Parameters
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