Composite
Part:BBa_K3088003
Designed by: Syailendra Karuna Sugito, Eko Ngadiono, Refael Alfa Budiman, Aditya Parawangsa Group: iGEM19_UI_Indonesia (2019-10-08)
DiphTox + RFP
This design is an improvement of the previous DiphTox (DT) (BBa_K2607000). We changed the Ribosomal Binding Site and added Red Fluorescent Protein (RFP) sequence following the DT protein. The addition of RFP may ease and improve post-transformation bacterial selection process in addition to antibiotic selection to prevent selecting bacteria that contains re-ligated plasmid.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 791
Illegal AgeI site found at 903 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |