Part:BBa_K2918008
Transcription Activator like Effector protein (TALEsp1)
Usage and Biology: Transcription Activator Like Effectors (TALE) proteins were originally discovered in a plant pathogenic bacterial genus Xanthomonas (Doyle, Stoddard et al. 2013). These proteins consists of repeats where 12th and 13th amino acids can vary, these are called the repeat variable diresidue (RVD). These RVDs have been shown to bind to DNA in a simple one-to-one binding code (Doyle, Stoddard et al. 2013):
Amino acids Target HD C NG T NN G NI A
The direct correspondence between amino acids allows scientists to engineer these repeat regions to target any sequence they want.
In our system we used the TALE protein as a repressor by engineering promoters to contain the binding site of this specific TALE protein.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 235
Illegal PstI site found at 2490 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 235
Illegal PstI site found at 2490 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 202
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 235
Illegal PstI site found at 2490 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 235
Illegal PstI site found at 2490
Illegal AgeI site found at 1229 - 1000COMPATIBLE WITH RFC[1000]
None |