Part:BBa_K3195003

His-tagged Cathepsin B

This Cathespin B from Branchiostoma belcheri tsingtauense is originally a kind of hydrolase in lysosome.In our project,it has been used to biodegrade microcystin,a kind of biotoxin synthesized by cyanobacteria.

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Usage and Biology

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Our BioBrick encodes a laccase from the fungus Trametes versicolor (PDB: 1GYC). Laccases are a class of multi-copper oxidases that fungi use to degrade lignin and be able to grow on wood [2]. Lignin is composed of a cluster of multiple phenolic groups, which are oxidized by laccases. With their natural affinity for phenolic-like structures, laccases have also been shown to degrade a large range of other phenolic compounds. For example, many reports show that laccases can be used to degrade pharmaceuticals - including estrogens, painkillers and antibiotics [3].

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 369
  Illegal XhoI site found at 958
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 463
  
• 1000
  COMPATIBLE WITH RFC[1000]