Coding

Part:BBa_K3098015

Designed by: Jiongyi He   Group: iGEM19_WHU-China   (2019-10-04)
Revision as of 09:31, 4 October 2019 by Registry (Talk | contribs)

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sfGFP+Avi-tag+His-tag

iGEM19_WHU-China added Avi-tag to C-terminal of sfGFP to confirm the function of BirA enzyme(BBa_K2485001). Avi-tag can be specifically recognized by BirA enzyme in vitro/vivo to attach a biotin. Furthermore, this part can be used to produce biotinylated sfGFP for its purification and other signal detection/amplification possibilities in future versatile platform.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 25
    Illegal AgeI site found at 148
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None