RBS
URBS
Part:BBa_K2918014
Designed by: TUDelft2019 Group: iGEM19_TUDelft (2019-09-24)
Universal RBS
Ribosomal Binding Site functional in both gram positives and gram negatives
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Usage and Biology
This ribosomal binding site has been made by the use of the RBS calculator 2.0 by Sen Yang et al. This RBS functions both in gram negative (E. coli) and gram positive (B. subtilis).
This part has been designed with Modular Cloning compatible overhangs (TACT - AATG). It makes use of the TypeIIs BsaI enzyme.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |