Composite
Medium p6

Part:BBa_K2918024

Designed by: TUDelft2019   Group: iGEM19_TUDelft   (2019-09-27)
Revision as of 10:55, 27 September 2019 by Hafsaflats (Talk | contribs)


Medium T7 promoter - Universal RBS - Φ29 DSB (p6) - WT T7 terminator

This part consists of a T7 promotor, a universal Ribosome Binding Site (RBS), a Coding DNA Sequence (CDS) coding for the DSB p6 and a Wild Type T7 terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 292
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 68
    Illegal BsaI.rc site found at 94

Overview

This protein is one of the 4 components needed for orthogonal replication

Characterization

Protein gel + Mass Spectrometry + transformation + sequencing(gotag check pcr)

Toxicity

[edit]
Categories
Parameters
None