Composite

Part:BBa_K2992042

Designed by: Yaseen Tengur   Group: iGEM19_Nottingham   (2019-10-01)
Revision as of 11:11, 1 October 2019 by YasTen (Talk | contribs)


Promoterless FAST reporter construct

Usage and Biology

This parts entry represents a promoterless FAST reporter construct. The construct comprises the fluorescent reporter gene FAST BBa_K2992000, coupled with the strong clostridial terminator TFad BBa_K2284012. FAST is one of the few fluorescent reporters available for effective use in anaerobic organisms. FAST is derived from Halorhodospira halophila and has been codon optimised for fluorescence studies in the genus Clostridium. In our project we couple FAST with the natural promoters of the BotR regulon thus linking reporter fluorescence with botulinum neurotoxin production. In doing so, we hoped to generate our surrogate host strain as a model for predicting neurotoxin production in foodstuffs following food manufacturing processes.

Characterisation

Data incoming.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 155

References

Street et al 2019. Update


[edit]
Categories
Parameters
None