Intermediate
Part:BBa_K3128016
Designed by: Melodie Dupre Group: iGEM19_Grenoble-Alpes (2019-09-18)
COMP fused with T25 subpart of Bordetella Pertussis adenylate cyclase under lactose promoter
This biobrick is composed of the external membrane OMPx protein with Y121F' mutation fused at its N-terminal end with a 54 aa GGS linker (BBa_K3128010) followed by the T25 subunit of the Bordetella Pertussis adenylate cyclase. This biobrick is under plac promoter in order to be activated when the cAMP messenger is present in the system.
This biobrick has a TAG codon (Y121F' mutation) which can be used to introduce any Non Natural Amino acid outside the cell (more informations).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1597
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |