Coding
Cyt1Aa Wit

Part:BBa_K2938007

Designed by: Assaf Vital   Group: iGEM19_BGU_Israel   (2019-09-28)
Revision as of 12:31, 28 September 2019 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Cyt1Aa With Strep Tag

Cyt1Aa Bti toxin with Strep-tag attached in the C- terminal in order to be able to detect the translated protein in western blot (using antibodies against the tag)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 419
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 419
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 717
    Illegal BamHI site found at 96
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 419
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 419
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None