Composite

Part:BBa_K3198007

Designed by: Chunyang Song   Group: iGEM19_NUS_Singapore   (2019-09-27)
Revision as of 07:37, 27 September 2019 by Registry (Talk | contribs)

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HicA-LuxABCDE

This part contains the toxin component of a type II toxin-antitoxin (TA) system. HicA is a probable translation-independent mRNA interferase. Bacterial lux operons encodes five enzymes involved in the light-generating pathway. LuxA and LuxB encode the two subunits of the bacterial luciferase, while the products of LuxC, LuxD and LuxE synthesise the substrate for the light emitting reaction, tetradecanal. The exact function of LuxG is unknown, and it appears to be non-essential for light emission, but its presence increases light output.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 549
    Illegal NheI site found at 1767
    Illegal NheI site found at 4733
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3731
    Illegal BamHI site found at 489
    Illegal BamHI site found at 1706
    Illegal XhoI site found at 4561
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1541
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 6120
    Illegal BsaI.rc site found at 3129
    Illegal SapI site found at 1523
    Illegal SapI.rc site found at 6445


[edit]
Categories
//awards/composite_part/nominee
Parameters
None