Reporter
Plac_NLuc

Part:BBa_K3128001:Design

Designed by: Lucas PINERO   Group: iGEM19_Grenoble-Alpes   (2019-09-15)
Revision as of 19:30, 15 September 2019 by Pinerol (Talk | contribs) (→‎Design Notes)


NanoLuciferase reporter for BACTH assay


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To do a BACTH assay the reporter gene need to :

Have a CAP inducible promoter : PLac

A strong RBS to optimize protein expression

A easly measurable protein : NanoLuciferase


595px-T--Grenoble-Alpes--Plasmid_PLacNanoLuc.png

Source

iGEM plate : Bba_J04450 and Bba_K1680009

References