Coding

Part:BBa_K3195003:Design

Designed by: JingYuan Liu   Group: iGEM19_SEU-Nanjing-China   (2019-09-23)
Revision as of 15:18, 23 September 2019 by Bread (Talk | contribs) (→‎Design Notes)


His-tagged Cathepsin B


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 369
    Illegal XhoI site found at 958
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 463
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Because Cathespin B gene is in eukaryotes，we optimized its condons for better prokaryotic expression.Considering the following seperation and purification,we add 6X His at its tail.In addition,we cut off the signal peptide.

Source

Cathespin B gene from Branchiostoma belcheri tsingtauense

References