Coding
EF-Tu

Part:BBa_K2916009

Designed by: Konstantinos Ragios   Group: iGEM19_EPFL   (2019-07-24)
Revision as of 15:24, 24 July 2019 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


EF-Tu protein equipped with a 6x HIS affinity tag

Leucyl-tRNA synthetase is a fusion protein that is used in the PURE and OnePot PURE cell-free systems.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1183
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 280
    Illegal AgeI site found at 685
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 430


[edit]
Categories
Parameters
None