Composite
Part:BBa_K2933281
Designed by: Weisi Wang Group: iGEM19_TJUSLS_China (2019-09-15)
RBS+Linker h+His+Linker f+ARL-1+T7 terminator
This part consists of RBS, protein coding sequence(His+Linker h+Sumo+Linker f+ARL-1) and T7 terminator,and the biological module can be build into E.coli for protein expression. This part can be prefaced with promoters of different strengths and types to regulate expression function.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 555
Illegal PstI site found at 669
Illegal PstI site found at 720 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 93
Illegal NheI site found at 985
Illegal PstI site found at 555
Illegal PstI site found at 669
Illegal PstI site found at 720 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 555
Illegal PstI site found at 669
Illegal PstI site found at 720 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 555
Illegal PstI site found at 669
Illegal PstI site found at 720 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |