![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K2951008:Design
Designed by: YU-RU LIN Group: iGEM19_CSMU_Taiwan (2019-09-19)
LysRS-Hemagglutinin fusion protein
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 275
Illegal NotI site found at 1256 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 864
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1845
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
For Hemagglutinin 1.Codon optimization: We optimized the sequence's codon usage for E.coli.
2.RFC 10 compatible: We checked for restriction sites for EcoRI, XbaI, SpeI, PstI and NotI in order to make it RFC compatible.
3.Deletion of transmembrane domain: To improve the solubility of hemagglutinin, we predicted the transmembrane domain with TMHMM Server v. 2.0.
Source
Influenza A virus (A/WSN/1933 TS61(H1N1) obtained from NCBI GenBank(CY010788.1).