![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K2933242
Designed by: Yongjie Li Group: iGEM19_TJUSLS_China (2019-09-15)
RBS a+Linker g+GST+Linker e+Fla.103
This part consists of RBS a, protein coding sequence(GST+Linker e+Fla.103). The blaFla.103 is separated from Flavobacterium sp. 103. The RBS and the protein coding sequence can be connected by linker g. The biological module can be build into E.coli for protein expression. This part can be prefaced with promoters of different strengths and types to regulate expression function.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 802
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 802
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1284
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 802
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 802
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 113
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Categories
Parameters
None |