Composite

Part:BBa_K2933241

Designed by: Yongjie Li   Group: iGEM19_TJUSLS_China   (2019-09-15)
Revision as of 09:30, 15 September 2019 by Registry (Talk | contribs)

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RBS a+Linker g+GST+Linker e+IMP-71

This part consists of RBS a, protein coding sequence(GST+Linker e+IMP-71), the RBS and the protein coding sequence can be connected by linker g. The biological module can be build into E.coli for protein expression. This part can be prefaced with promoters of different strengths and types to regulate expression function.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1450
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1450
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1450
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1450
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 113


[edit]
Categories
Parameters
None