Deleted
Sample Discontinued
Experience: None
Not Used
Get This Part
Composite

Part:BBa_K2933230

Designed by: Yongjie Li   Group: iGEM19_TJUSLS_China   (2019-09-15)
Revision as of 08:29, 15 September 2019 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


RBS a+Linker g+GST+Linker e+CPS-1

This part consists of RBS a, protein coding sequence(GST+Linker e+CPS-1), the RBS and the protein coding sequence can be connected by linker g. The biological module can be build into E.coli for protein expression. This part can be prefaced with promoters of different strengths and types to regulate expression function.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1263
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1263
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1545
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1263
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1263
    Illegal AgeI site found at 1164
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 113


[edit]
Categories
Parameters
None