Composite

Part:BBa_K2933215

Designed by: Pengqian Li   Group: iGEM19_TJUSLS_China   (2019-09-15)
Revision as of 13:03, 23 September 2019 by Pengqian (Talk | contribs)


T7 promoter+RBS b+Linker h+His+Linker f+BCll-194+T7 terminator

This part consists of T7 promoter, RBS and protein coding sequence(His+Linker f+NDM-23),and the biological module can be built into E.coli for protein expression.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 47
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 169
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 47
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 47
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology=

This composite part is made up with seven basic parts, the His tag, T7 promoter, RBS and our target protein BcII-194. It encodes a protein which is BcII-194 fused with His tag. The fusion protein is about 28.1 kD. In order to gain the highly purified target protein, we add His tag in N-terminal of BcII-194. It is convenient for us to purify our target protein.

Molecular cloning

First,we obtained BcII by PCR.

BcII-194 PCR.jpeg
Figure 1. The PCR result of BcII.

[edit]
Categories
Parameters
None