Composite

Part:BBa_K2933225

Designed by: Yongjie Li   Group: iGEM19_TJUSLS_China   (2019-09-15)
Revision as of 08:15, 15 September 2019 by Registry (Talk | contribs)

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RBS a+Linker g+GST+Linker e+ElBlaII

This part consists of RBS a, protein coding sequence(GST+Linker e+ElBla2-1), the RBS and the protein coding sequence can be connected by linker g. ElBla2-1 is a MBLs isolated from B that we temporarily named it as ElBla2-1. The biological module can be build into E.coli for protein expression. This part can be prefaced with promoters of different strengths and types to regulate expression function.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1210
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 113


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Categories
Parameters
None