RNA

Part:BBa_K581005

Designed by: Qin Xiao   Group: iGEM11_Peking_S   (2011-09-26)
Revision as of 05:06, 23 September 2019 by Lowxizhi (Talk | contribs)

Pc+sgrS(wt)+Terminator (small RNA regulator, conjugate part of ptsG(wt))

SgrS(sugar transport-related sRNA)(wt) is a small RNA regulator that help cells recover from glucose-phosphate stress by base pairing with ptsG(wt)[1] mRNA. SgrS regulates ptsG mRNA by short, imperfect base-pairing interactions; as a result, the expression of PtsG is repressed.

Teppei Morita et.al’ s work suggests that two mutations (C85G and C87G) in ptsG mRNA could completely impair the ability of SgrS to downregulate its expression, while compensatory mutations of SgrS (G178C and G176C) restore the gene silencing ability. These results indicate that it is the base pairing of the two RNAs rather than particular nucleotides that is important for SgrS action. They have also illustrated that sequence outside this region, even though complementary, is rather dispensable for the efficient silencing (Kawamoto et al., 2006). This makes mutant ptsG/SgrS pairs orthogonal to genetic context of the host cell. Therefore we choose this couple of conjugate mRNA/sRNA as the foundation of our comparator device design.

SgrS(wt) in this part will be constitutively expressed in E.coli after transformation processing.

                 Mechanism.png
                Fig.1 Sequence alignment of wildtype ptsG/SgrS pair and its mutant complementary pairs. 


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]







Following is a contribution to this part, by the NUS_Singapore 2019 team.


Group

NUS_Singapore 2019

Author

Sheena Chan

Summary and Uploads

[edit]
Categories
//chassis/prokaryote/ecoli
//regulation/negative
Parameters
device_typepost-transcriptional regulators of gene expression
familyHfq-binding small RNA
functiondestabilzes ptsG(wt) mRNA
originE.coli K-12 strain
target5' Untranslated Regions