Part:BBa_K2933143
His+Linker f+beta-lactamase II [Erythrobacter litoralis HTCC2594]
This part encodes the fusion protein of His tag and beta-lactamase II to promote the expression and purification of beta-lactamase II [Erythrobacter litoralis HTCC2594].
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 51
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 570
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
This composite part is made up with three basic parts, the HiS tag, the thrombin restriction site and our target protein Elbla2-1. It encodes a protein which is Elbla2-1 fused with His tag. The fusion protein is about 27.5 kD. It is convenient for us to purify our target protein.
Molecular cloning
First, we used the vector pET28B-Sumo to construct our expression plasmid. And then we converted the plasmid constructed to E. coli DH5α to expand the plasmid largely.
Figure 1. The PCR result of Elbla2-1.
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