Composite

Part:BBa_K2933222

Designed by: Pengqian Li   Group: iGEM19_TJUSLS_China   (2019-09-15)
Revision as of 05:19, 15 September 2019 by Registry (Talk | contribs)

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T7 promoter+RBS b+Linker h+His+Linker f+Fla.103+T7 terminator

This part consists of T7 promoter, RBS and protein coding sequence（His+Linker f+Fla.103），and the biological module can be built into E.coli for protein expression.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 47
    Illegal PstI site found at 280
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 169
    Illegal PstI site found at 280
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 762
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 47
    Illegal PstI site found at 280
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 47
    Illegal PstI site found at 280
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None