![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K2933213
Designed by: Pengqian Li Group: iGEM19_TJUSLS_China (2019-09-15)
T7 promoter+RBS b+Linker h+His+Linker f+JOHN-1+T7 terminator
This part consists of T7 promoter, RBS and protein coding sequence(His+Linker f+JOHN-1),and the biological module can be built into E.coli for protein expression.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 47
Illegal PstI site found at 280 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 169
Illegal PstI site found at 280 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 47
Illegal PstI site found at 280 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 47
Illegal PstI site found at 280 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 804
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Categories
Parameters
None |