Deleted
Sample Discontinued
Experience: None
Not Used
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Composite

Part:BBa_K2933254

Designed by: Ruoming Sun   Group: iGEM19_TJUSLS_China   (2019-09-15)
Revision as of 11:20, 15 September 2019 by Registry (Talk | contribs)

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RBS b+Linker h+His+Linker a+Sumo+Linker b+JOHN-1+T7 terminator

This part consists of RBS, protein coding sequence(His+Linker a+Sumo+Linker b+JOHN-1) and T7 terminator,and the biological module can be build into E.coli for protein expression. This part can be prefaced with promoters of different strengths and types to regulate expression function.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 298
    Illegal PstI site found at 473
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 298
    Illegal NheI site found at 75
    Illegal NheI site found at 1152
    Illegal PstI site found at 473
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 298
    Illegal BglII site found at 187
    Illegal BamHI site found at 386
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 298
    Illegal PstI site found at 473
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 298
    Illegal PstI site found at 473
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 997


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Categories
Parameters
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