Coding
TmEnc

Part:BBa_K3111002

Designed by: Matas Deveikis   Group: iGEM19_UCL   (2019-09-10)
Revision as of 15:58, 10 September 2019 by Matas deveikis (Talk | contribs)


T. maritima encapsulin from BBa_K2686001 (no stop codon)

Modification to Team EPFL 2018 part BBa_K2686001 with addition of a StrepII tag to purify assembled encapsulin without heat treatment. Enterokinase site allows for post-purification removal of the tag.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 77
    Illegal BglII site found at 441
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 375
    Illegal SapI.rc site found at 406


[edit]
Categories
Parameters
None