Protein_Domain
CHAP
Part:BBa_K3182004
Designed by: Leo Juhlin Group: iGEM19_Linkoping_Sweden (2019-07-13)
CHAP(K)
Staphylococcus phage endolysin, designed to target Staphylococcus aureus. This part was designed to be assembled with BBa_K3182010.
Bacteriophage endolysins digest the peptidoglycan wall of bacteria. The LysK is an endolysin targeting gram + bacteria. It has two catalytical domains known as CHAP (cysteine-and histidine depentent amidohydrolase/peptidase) and a central amidase-2 domain together with a cell binding domain. The CHAP domain which is the truncated part expressed in this year’s LiU iGEM (more specifically CHAP C1-C162) team still has lytic activity against staphylococcus. The C1 to C162 amino acids are required for full endopeptidase activity.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |