Regulatory

Part:BBa_K2992001

Designed by: Yaseen Tengur   Group: iGEM19_Nottingham   (2019-09-03)
Revision as of 17:06, 12 September 2019 by YasTen (Talk | contribs) (→‎Usage and Biology)


PntnH

Promoter region for the non-toxic nonhemmagluttinin component of the neurotoxin gene cluster in Clostridum botulinum.

Usage and Biology

Pntnh is found upstream of the 5'-UTR and RBS of the non-toxin nonhemmagluttinin component (ntnh) of the neurotoxin gene cluster in C. botulinum. This promoter was used to drive expression of of the toxin regulator botR and our chosen reporter genes of interest which have been placed under the control of the neurotoxin and neurotoxin-associated promoters. Doing so allows us to link volatile reporter production with neurotoxin production following food manufacturing processes.

Characterisation

Data incoming.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Raffestin et al., 2005 (update)

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Categories
Parameters
None