Coding

Part:BBa_K3168003:Design

Designed by: Eva Hanckmann, Harm van der Veer, Claire Michielsen   Group: iGEM19_TU_Eindhoven   (2019-09-12)
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SmallBitNanoLuc


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 85
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A flexible (GGS)5 linker is located in front of the small bit to enable the formation of fusion proteins. A strep tag is also included at the end for protein purification.


Source

deep-sea shrimp derived luciferase

References