![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K2933102
Designed by: Dongxu Li Group: iGEM19_TJUSLS_China (2019-09-06)
His+Linker a+Sumo+Linker b+SPG-1
This part encodes the fusion protein of His-Sumo tag and SPG-1 to promote the expression and purification of target protein(SPG-1).
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 256
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 256
Illegal NheI site found at 33 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 256
Illegal BglII site found at 145
Illegal BamHI site found at 344 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 256
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 256
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//awards/composite_part/nominee
None |