Coding

Part:BBa_K2992003

Designed by: Yaseen Tengur   Group: iGEM19_Nottingham   (2019-09-05)
Revision as of 13:38, 5 September 2019 by YasTen (Talk | contribs)


ctfA from C. botulinum

ctfA encodes the A sub-unit of Butyrate-acetoacetate CoA-transferase complex.


Usage and Biology

The CtfAB complex is central to to the production of biotechnologically relevant solvents in C. acetobutylicum such as acetone and butanol. The complex catalyses the conversion of acetoacetyl-CoA to acetoacetate concomitant with the transfer of CoA to acetate and butyrate. the CtfAB complex is a core component of our acetone production pathway to validate the use of volatile reporters to predict botulinum toxin production following food manufacturing processes. We have tested both C. acetobutylicum and C. botulinum-derived ctfAB.

Characterisation

Data incoming.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterisation

Incoming


[edit]
Categories
Parameters
None