Coding
Part:BBa_K2927003
Designed by: KUO, PEI-CHING Group: iGEM19_CCU_Taiwan (2019-08-28)
Maltose-binding protein (MBP)
MBP is the sequence that coding for the maltose-binding protein. Maltose binding protein can be trapped by the MBP column after the TEV enzyme cut TEV cutting site of composition (10X His-MBP-TEV-Cas12a). For the function, the Cas12a protein will exist in the solution. The MBP is used by E.coli.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 360
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 58
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Categories
Parameters
None |