Generator

Part:BBa_K3174002

Designed by: iGEM19_Austin_UTexas   Group: iGEM19_Austin_UTexas   (2019-08-08)
Revision as of 20:28, 8 August 2019 by Dbhat (Talk | contribs) (→‎Usage and Biology)


Blue Fluorescent Protein with Strong Promoter and Strong RBS

This part has the blue fluorescent protein (mTagBFP) gene inserted behind a strong promoter and strong ribosome binding site.


Usage and Biology

UT Austin iGEM 2019 Team transformed this part into the E.coli DH10B burden monitor No part name specified with partinfo tag. and used it as a standard with which cellular burden could be quantified (by means of a GFP expression vs growth rate model) for other biobricks that had been similarly transformed into the burden strain.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
functionBurden: 17.8 +/- 1.6%