Coding

Part:BBa_K2982001:Design

Designed by: Tsang Hoi Yeung, Ocean   Group: iGEM19_HK_GTC   (2019-08-03)
Revision as of 12:45, 3 August 2019 by Registry (Talk | contribs)

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Coding sequence for W159H/S238F IsPETase double mutant


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 348
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 304
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 348
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 348
    Illegal AgeI site found at 627
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The double mutant narrows active site to make it more TfCutinase-like which increases its activity on PET plastic


Source

Sequence of double mutant in 'Characterization and engineering of a plastic-degrading aromatic polyesterase', PNAs, 2018.

References